SG97A平衡致死系统的构建及其初步应用

doi:10.11843/j.issn.0366-6964.2015.12.016

Abstract

Abstract:

The aim of this study was to delete asd gene of Salmonella gallinarum (SG) attenuated vaccine strain 97A and construct bivalent live vaccine based on host-vector balanced lethal system to express F protein of Newcastle disease virus(NDV).Two DNA fragments flanking asd gene as allele were amplificated by PCR and asd gene was replaced by chloramphenicol resistance(Cm^R) gene to construct the suicide plasmid pGMB151Δasd：：Cm^R.After conjugation，the recombiant plasmid was transformed into SG97A from E.coli χ7213，SG97AΔasd：：Cm^R was reversely screened out under the selectable pressure of chloramphenicol，DAP-dependent strain was named SG97AΔasd.Vector-host balanced lethal system was construct by pYA3334 containing asd gene being transferred into SG97AΔasd and to expressed F protein of NDV.SG97AΔasd was successfully constructed by allelic exchanges，its growth was DAP-dependent.Vector-host balanced lethal system was constructed by pYA3334 being transferred to SG97AΔasd and could express F protein of NDV and also induce specific antibodies in infected chickens.Vector-host balanced lethal system was constructed successfully based on attenuated Salmonella SG97AΔasd in this study and could express F gene of NDV as foreign gene to induce special antibodies in animal model，it laid a foundation for bivalent live vaccine based on attenuated Salmonella.

CLC Number:

S852.612

GENG Shi-zhong，CHEN Xiao-juan，PAN Zhi-ming，JIAO Xin-an. Construction and Preliminary Application of Balanced Lethal System Based on Attenuated Salmonella SG97A[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(12): 2243-2250.

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References

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Construction and Preliminary Application of Balanced Lethal System Based on Attenuated Salmonella SG97A

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